Heterologous overexpression, purification and mass spectrometric analysis of the human tachykinin NK2 receptor and

103 INTRODUCTION 104 MATERIALS AND METHODS 106 Materials 106 Construction of the recombinant expression vector 106 Exchange of the rare codons present in the hNK2R for expression in E.coli 107 Expression of the MBP-hNK2R in E.coli 110 Analysis of the expression of the MBP-hNK2R fusion protein in E.coli 111 Purification of the MBP-hNK2R fusion protein 111 Cleavage of the MBP-hNK2R fusion protein and isolation of the hNK2R 112